Electrospray ionization mass spectrometry esi ms has demonstrated utility for the detection and study of weakly bound noncovalent complexes including protein interactions with inhibitors cofactors metal ions carbohydrates other peptides and proteins enzyme substrate pairings and nucleic acid complexes from the measurement of molecular mass of the intact complex and the individual binding partners the binding stoichiometry can be derived in many examples the . Ionization technique which is coupled with mass electrospray ionization mass spectrometry requires that a molecule be of a fairly large mass the instrument has a small mass range that it is able to detect so therefore the mass of the unknown injected sample can easily be electrospray ionization mass spectrometry applied technology aug 26. Mass spectrometry has four essential functions which are ionization separation filtering detection and analysis these functions are coordinated by the following components the ion source mass analyzer detector and software when combined with chromatography such as liquid or gas chromatography the potential for ms to identify and quantitate analytes of interest increases dramatically
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